RUB Research School

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Author
Name Moreno Wasielewski, Laura
Research field Ionotropic glutamate receptors
Career stage doctoral researcher
Home university/institution Ruhr-Universität Bochum (RUB)
Department/Research unit at home university/institution Biology and Biotechnology
Chair/Working group at home institution Cellular Neurobiology

International activity
Country United States
Location New York
University Weill Cornell Medical College
Fund Research School PR.INT
Type of activity research stay
Period starts 05-10-2025
ends 26-10-2025
Keywords Molecular Neurobiology, Single-Molecule Imaging, Protein Complexes, Ion Channels, Glutamate Receptors, Single-molecule pull-down (SiMPull), Stoichiometry, Assembly
Report The goal of my PhD project is to analyze the functional properties and assembly of iGluR heteromers. In this context I want to analyze the stoichiometries of different glutamate receptor subunit combinations using the single-molecule pull-down (SiMPull) technique in combination with a photobleaching step analysis. During my research stay, I was able to learn this unique technique in the lab of a world-leading expert in this field, Prof. Dr. Joshua Levitz, who has already successfully extended this technique to various glutamate receptors. Overall, I was able to learn the necessary tools to establish this method in our lab, gained new ideas for my PhD project, and could open the doors for further cooperations.
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